Linked Michaelis-Menten type models for methanogenesis data

 

Technical details and software relevant to the report

 

“A stable isotope titration method for measurement of the contribution of acetate and carbon dioxide reduction to methane production”

 

By N.D. Gray, J.N.S. Matthews and I.M. Head

 

University of Newcastle upon Tyne

 

 

The above report discusses the fitting of a Michaelis-Menten model to the rate of production of 13CH4 produced in anaerobic microcosms titrated with varying amounts of 13C labelled substrate, together with a linked model for the amount of 12CH4 also produced.  This page gives access to some details of the technical statistical issues behind the method and also provides a program for fitting the model.

 

 

 

The program is the same one that was used to calculate the estimates in the above report.  It has been checked carefully for this purpose with this dataset.  No guarantee is given regarding the correctness of the answers the program produces.  The FORTRAN code may be modified by the user as they wish.  While the authors would be grateful to hear of any genuine errors users believe they have encountered, they give no undertaking to be able to fix these and cannot give advice on amendment or development of the software.